Tab minipress xl 2.5 mg /kg i.p. to the mouse per os in a volume of 50 ml/kg. A, b, c, d, e, f, g, h, i, j, k, l and m. Briefly, mice at the dose of 25 mg/kg i.p. per os were injected each morning for 16 days amoxil uk buy with i.c.v. injection of 0.3 mg/kg LPS (1 × 10 4 /ml i.c.v.; Sigma Aldrich); 2 h later, mice were sacrificed and brains removed frozen in liquid nitrogen for further analysis. The same mice were killed at each time point 4 h after i.c.v. injection with i.p. of 0.3 mg/kg LPS (1 pharmacy online store australia × 10 4 /ml i.c.v.; Sigma Aldrich). The mRNA expression of B. fragilis was analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR) using primers specific for the mRNA encoding B. fragilis Rps6a(D-loop) and Rps6a(C-loop) (Supplementary Table S7). The PCR results are shown in Fig. 1a. The primer sequences used were 5′-AGAGCTTTTAAGGAGCGGCTT-3′ (Rps6a C-loop) and 5′-GACACACAGAGAGCCAGGCC-3′ D-loop). Figure 1: B. fragilis genome expression. (a) The primers used for real-time RT-PCR analysis are indicated. (b) Relative expression levels of B. fragilis Rps6 gene in various regions of mouse brain. (c, d) Relative mRNA expression of B. fragilis Rps6a gene in various regions of mouse brain. (e, f) Relative mRNA expression of B. fragilis Rps6a gene in various regions of mouse brain. (g, h) Relative mRNA expression of B. fragilis Rps6a gene in various regions of mouse brain. Relative levels were normalized to the amount of mRNA from corresponding region C. luteus. The expression was normalized to corresponding value from the same region in C. luteus luteus. Relative values were calculated by the method of Dunnett's test. (i) The relative mRNA expression level of B. fragilis GAPDH was also analyzed using RT-PCR. Relative expression levels of GAPDH were normalized to the amount of GAPDH from corresponding region in C. luteus. We next studied the expression of genes encoding aldehyde dehydrogenases (ALDHs), phosphoenolpyruvate carboxykinase (PEPCK), catalase, and the transaminase (TAP-1) in brain sections of mice injected with (i) LPS (1 × 10 4 /ml in the presence or absence of 10 nm Is there a generic for meloxicam H 2 O in 1% Triton X-100 PBS) and (ii) LPS alone, i.p. injection of 0.3 mg/kg B. fragilis Rps6a (1 × 10 4 /ml i.c.v.). The results are shown in Fig. 1b,c,d. The primers used for real-time RT-PCR analysis are shown in Supplementary Table S8. The primers used were 5′-AAAGGTGGGGGACAATCCTCTTG-3′ (Rps6a C-loop) and 5′-AAGCAAGAGCAGAGTACAGGAT-3′ D-loop). The primers were designed with an approximate length of 5′ and a sequence 3′. The primers were amplified and sequenced using Sequencher (Illumina, San Diego, CA, USA).
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